U-2 OS-Luc cell line

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Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Cell Monoclonal Culture Medium

Monoclone Genotype Validation Kit

U-2 OS-Luc Order

Catalog#

YC-C030-Luc-P

Size

1*10^6

Transfection Culture Medium

Transfection Culture Medium-5A

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Datasheet

Order

Detailed Product Information

Product category

Luciferase Stable Cell Lines

cell name

U-2 OS-Luc

Morphology

Epithelial-like, adherent

Passage ratio

1:3~1:5

Species

Human

Reporter gene

Luciferase

Resistance gene

puro

Construction method

Lentivirus

Mycoplasma test

negative

Culture method

90% McCOY's 5A+10%FBS

Accession

CVCL_C8Y6(Cellosaurus)

Luciferase validation

Sample	Compound pore 1	Compound pore 2	Compound pore 3	Average	Expressed multiple
U-2 OS-Luc	6736	6955	8663	7451.333	22.626
U-2 OS	340	264	384	329.333	22.626

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:U-2 OS			STR Info (Cell bank) Cell Line:U-2 OS
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	11		11	11
D13S317	13	13		13	13
D7S820	11	12		11	12
D16S539	11	12		11	12
VWA	14	18		14	18
TH01	6	9.3		6	9.3
AMEL	X	X		X	X
TPOX	11	12		11	12
CSF1PO	13	13		13	13

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use luciferase gene expressing lentivirus constructed by Ubigene to infect the U-2 OS cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a U-2 OS-Luc cell line that can maintain long-term, stable and high expression.

Product Advantage

Easy to use: Luciferase Stable Cell Line in our cell bank can stably express luciferase. It can be directly used for in vivo injection to detect the tumorigenicity of cells.
01
Sensitive accurate：Luciferase Stable Cell Line in our cell bank have high specificity and high sensitivity, enables high imaging quality, and its luminescence intensity can be accurately quantified.
02
High-quality cell: Our Luciferase Stable Cell Lines have been optimized and have low passages, high activity and good cell condition, applicable for detection or tumorigenesis experiment.
03

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